In vitro anti-HSV-2 activity and mechanism of action of proanthocyanidin A-1 from Vaccinium vitis-idaea

✍ Scribed by Hua-Yew Cheng; Ta-Chen Lin; Chien-Min Yang; Den-En Shieh; Chun-Ching Lin

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 120 KB
• Volume: 85
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/jsfa.1958

✦ Synopsis

Abstract

The aim of this study was to investigate the in vitro anti‐HSV‐2 activity and mechanism of action of proanthocyanidin A‐1, a compound isolated from Vaccinium vitis‐idaea Linn (Ericaceae). The results demonstrated that proanthocyanidin A‐1 exhibited anti‐HSV‐2 activity. The IC~50~ value for the XTT assay was 73.3 ± 14.5 µM and the IC~50~ and IC~90~ values for the plaque reduction assay were 41.9 ± 2.0 and 62.8 ± 6.3 µM respectively. Proanthocyanidin A‐1 showed no cell cytotoxic effect at concentrations that blocked HSV‐2 infection, with a CC~50~ value of 282.1 ± 27.5 µM. The mechanism studies demonstrated that proanthocyanidin A‐1 did not reduce viral infectivity but inhibited viral attachment and penetration and affected the late stage(s) of HSV‐2 infection. It was concluded that proanthocyanidin A‐1 suppressed HSV‐2 infection through many modes of action and thus merits further investigation. Copyright © 2004 Society of Chemical Industry