In vitro andin vivo digestion of collagen covalently immobilized onto the silicone surface

✍ Scribed by Okada, Toshiyuki ;Ikada, Yoshito

• Book ID: 102873177
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 641 KB
• Volume: 26
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.820261204

✦ Synopsis

In order to study the in v i m digestion of immobilized collagen and gelatin, these proteins labeled with '''1 or fluorescein isothiocyanate (FITC) were covalently immobilized onto silicone surfaces, which were grafted with acrylic acid to introduce carboxyl groups, and implanted subcutaneously in rats and mice. When the proteins were labeled with FITC, the amount of proteins immobilized decreased with the increase of the number of FITC molecules conjugated with the protein molecule. In the wet state, FITC conjugated with the proteins was less stable than '=I. Approximately half of the amount of the immobilized proteins was digested in vim within the first week and until 5 weeks after implantation the pro-teins were gradually digested. At that time, the amount of the proteins remaining on the silicone surface ranged from 0.6 to 1.0 pg/cm2, which was several times larger than the amount of an assumed monolayer adsorption of proteins. Even after 15 weeks, the amount of proteins remaining on the silicone was almost the same as after 5 weeks. No significant difference in digestion was observed between collagen and gelatin, regardless of the labeling agent. Because of less stability and easier handling of FITC and higher stability and more difficult handling of '=I, FITC seems more suitable for short-term and I2'I for long-term studies.