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In vitro and in vivo recombination-related reactions of Escherichia coli recA protein and glucosyl-hydroxymethyl-deoxycytidine DNA

✍ Scribed by Yonesaki, Tetsuro ;Minagawa, Teiichi

Publisher: Springer
Year: 1988
Tongue: English
Weight: 348 KB
Volume: 213
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00339630

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✦ Synopsis

Recombination of T4 phage is not controlled by the host recA gene but by an analogous phage gene, uvsX. We have tested the hypothesis that recA protein is inactive in T4-infected cells because it is unable to catalyze reactions involving single stranded DNA containing glucosyl-hydroxylmethyl-deoxycytidine. We found, however, that with modified and unmodified deoxycytidine containing DNAs, uvsX protein and recA protein catalyze in vitro reactions related to DNA recombination, but in T4-infected cells recA protein fails to promote strand transfer of DNA which contains unmodified deoxycytidine.

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