In vitro and in vivo gene transfer with poly(amino acid) vesicles
✍ Scribed by Maureen D Brown; Alexander I Gray; Laurence Tetley; Ana Santovena; Judith Rene; Andreas G Schätzlein; Ijeoma F Uchegbu
- Book ID
- 113690405
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 868 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0168-3659
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## Abstract ## Background Polyethylenimine (PEI) is toxic although it is one of the most successful and widely used gene delivery polymers with the aid of the proton sponge effect. Therefore, development of new novel gene delivery carriers having high efficiency with less toxicity is necessary. #
We explored poly(4-vinylimidazole) (P4V) as a nonviral gene carrier. We show that P4V can form DNA condensates of small size (<110 nm) using a dye-exclusion assay with ethidium bromide and dynamic light scattering, and that the complexes form in a pHsensitive manner, due to the amphotericity of the