In vitro and in vivo comparisons of staphylococcal biofilm formation on a cross-linked poly(ethylene glycol)-based polymer coating
✍ Scribed by Isabel C. Saldarriaga Fernández; Henny C. van der Mei; Steve Metzger; David W. Grainger; Anton F. Engelsman; M. Reza Nejadnik; Henk J. Busscher
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 391 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1742-7061
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✦ Synopsis
Poly(ethylene glycol) (PEG) coatings are known to reduce microbial adhesion in terms of numbers and binding strength. However, bacterial adhesion remains of the order of 10 4 cm À2 . It is unknown whether this density of bacteria will eventually grow into a biofilm. This study investigates the kinetics of staphylococcal biofilm formation on a commercially produced, robust, cross-linked PEG-based polymer coating (OptiChem Ò ) in vitro and in vivo. OptiChem Ò inhibits biofilm formation in vitro, and although adsorption of plasma proteins encourages biofilm formation, microbial growth kinetics are still strongly delayed compared to uncoated glass. In vivo, OptiChem Ò -coated and bare silicone rubber samples were inserted into an infected murine subcutaneous pocket model. In contrast to bare silicone rubber, OptiChem Ò samples did not become colonized upon reimplantation despite the fact that surrounding tissues were always culture-positive. We conclude that the commercial OptiChem Ò coating considerably slows down bacterial biofilm formation both in vitro and in vivo, making it an attractive candidate for biomaterials implant coating.