## Abstract cAMP, dbcAMP, cCMP, cGMP, theophylline and caffeine caused reversible melanosome dispersion within 5 minutes at 10 mM in the dermal melanophores of the black goldfish, __Carassius auratus__ L. cTMP, cUMP, 5β²βAMP, 5β²βCMP, 5β²βGMP, 5β²βTMP, and 5β²βUMP did not produce melanosome dispersion o
In vitro analysis of gene depression in goldfish choroidal melanophores
β Scribed by Kajishima, Takao
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 538 KB
- Volume
- 191
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
β¦ Synopsis
Abstract
The common goldfish depigments its larval choroidal melanophores as well as the skin melanophores for about two to three months following hatching. Thereafter it begins to show a characteristic xanthic coloration. This depigmentation is under the control of depigmentation genes Dp~1~ and Dp~2~. The larval choroidal melanophores of the common goldfish were cultured in vitro for more than four months without showing any sign of depigmentation. During the same period the skin and choroidal melanophores in the control fish had completely depigmented. The addition of thyroid hormone and ACTH to the culture medium had no effect on depigmentation. The mechanisms of depigmentation in the goldfish are discussed.
π SIMILAR VOLUMES
The use of in vitro gene reporter assays is becoming increasingly widespread in biology and particularly in drug metabolism, where the need for rapid screening of novel compounds is a driving factor. There is, however, little standardization of technique in the control of such assays, nor in the int
## Abstract The study examined the feasibility of screening for hepatotoxicity by an __in vitro__ gene expression analysis using rat primary hepatocytes and Affymetrix Rat Toxicology U34 arrays. Hepatocytes were exposed for 6 or 24 h to eight drugs, with different mechanisms of hepatotoxicity, at o
## Abstract Isolated tissues from the land snail __Otala lactea__ were used to examine the relationship between protein kinase activity and phosphorylationβinduced changes associated with metabolic depression. Hepatopancreas and foot muscle were removed from active and estivating land snails and in
## Abstract Rat primary renal cortical tubular cells were exposed to seven test substances, some with, and some without, known direct renal tubular cell toxicity. Cells were exposed to the substances at either oneβthird or oneβtenth of the TC~50~ for cytotoxicity for 6 h or 24 h, so as not to induc