๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

In vitro 32P-labelling of viroid RNA for hybridization studies

โœ Scribed by V.I. Negruk; L.K. Grill; J.S. Semancik


Book ID
119113923
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
394 KB
Volume
1
Category
Article
ISSN
0166-0934

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


Detection of avocado sunblotch viroid (A
โœ Arie Rosner; Sara Spiegel; Miriam Alper; Moshe Bar-Joseph ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Springer ๐ŸŒ English โš– 549 KB

RNA was extracted from plants infected with avocado sunblotch viroid (ASBV) and was analyzed by electrophoresis in polyacrylamide gel. The ASBV related fraction was eluted from the gel, labelled with [(32)P] using polynucleotide kinase and used as a probe for hybridization with a purified ASBV-RNA p

Determination of 2โ€ฒ-O-methyl groups in 3
โœ I. Klein; J. Klootwijk ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 742 KB

A two-dimensional method for the detection of ribose methyl groups in 32P-labeled RNAs is described which is suitable for both qualitative and quantitative purposes. The method involves fractionation of an alkaline hydrolysate of the RNA by two-dimensional electrophoresis of the digest on DEAEcellul