In vitro 11-ketotestosterone and 17α,20β-dihydroxy-4-pregnen-3-one production by testicular fragments and isolated sperm of rainbow trout,Salmo gairdneri

✍ Scribed by Ueda, Hiroshi ;Kambegawa, Akira ;Nagahama, Yoshitaka

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 428 KB
• Volume: 231
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402310318

✦ Synopsis

Two different testicular tissue preparations, testicular fragments and isolated sperm were incubated in the presence or absence of chumsalmon gonadotropin (SGA, lpg/ml), 17a-hydroxyprogesterone (17a-OHprog, 0.1 pg/ml) and testosterone (T, 0.1 pg/ml). 11-Ketotestosterone (11-keto T) and 17a,20P-dihydroxy-4-pregnen-3-one (17a,20/3-diOHprog) in the incubation medium were measured by specific radioimmunoassay. Incubation of testicular fragments with SGA, 17a-OHprog or T resulted in a highly significant increase in 11-keto T levels in the incubation medium. Sperm preparations did not produce 11-keto T under any incubation condition. Both testicular fragments and sperm preparations produced large amounts of 17a,2(@-diOHprog when incubated with 17a-OHprog. SGA slightly stimulated 17a,20P-diOHprog by testicular fragments but not by sperm preparations. These results suggest that different testicular elements are the sites of synthesis of 11-keto T and 17a,20@-diOHprog. Furthermore, it is suggested the involvement of sperm in the production of 17a,2OP-diOHprog in the testis of spermiating rainbow trout.