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In the procurement of stable 99mTc labeled protein using bifunctional chelating agent

✍ Scribed by Yasushi Arano; Akira Yokoyama; Yasuhiro Magata; Kazuko Horiuchi; Hideo Saji; Kanji Torizuka

Publisher
Elsevier Science
Year
1986
Weight
556 KB
Volume
37
Category
Article
ISSN
0883-2889

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✦ Synopsis

Recently, technetium-99m (99mTc) labeling of proteins through the use of a bifunctional chelating agent (BCA) has been reported. In previous work, a BCA containing a di(N-methylthiosemicarbazone) (DTS) moiety for 99mTc has offered good potential; however, conjugation with bulky proteins has induced some steric interference in the 99mTc labeling step using the stannous chloride method. In order to minimize this effect, changes in the hydrocarbon chain length (spacer) between the DTS and the protein binding site (carboxyl group), as well as the chemical state of the reducing agent, stannous chloride, were considered of interest. DTS molecules with variable spacer length (n = 0, 2, 4) were synthesized, coupled with human serum albumin and labeled with 99mTc. Also, stannous chloride prepared in different media was evaluated. Validity of the present approach is tested and discussed.

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