In situ studies of subcellular particles immobilized in sucrose-acrylamide density gradients

✍ Scribed by Edward W. Khandjian

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 600 KB
• Volume: 77
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90252-4

✦ Synopsis

In order to study subcellular particles, I have applied a method to immobilize the content of a sucrose density gradient with a&amide after centrifugation. The different zones in the gels were localized and analyzed by specific stains and enzymatic, histochemical tests. This method allows the demonstration of several specific zones which could not be determined by traditional procedures. Moreover, the method requires only a few milligrams of material and can also be applied to other purposes such as autoradiography of gels containing minute amounts of radioactive products which could not be detected in a scintillation counter.

METHODS

Strain and Culture

Neurospora craSSa (strain 853 A obtained from the Fungal Genetics Stock, Humbolt State College, Arcata, Calif.) was grown for 24 hr in Vogel's medium N. For details see Ref. (2).

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