✦ LIBER ✦

In-situ seed production after pollination with in-vitro-matured, isolated pollen

✍ Scribed by R. M. Benito Moreno; F. Macke; A. Alwen; E. Heberle-Bors

Book ID: 104753053
Publisher: Springer-Verlag
Year: 1988
Tongue: English
Weight: 957 KB
Volume: 176
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00392439

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✦ Synopsis

Immature tobacco (Nicotiana tabacum L.) pollen has been isolated from anthers in three distinct stages of development, including the microspore stage. In in-vitro cultures, fully functional, mature pollen was obtained. In a germination medium, this pollen produced pollen tubes. After application to stigmas in situ, the in-vitro-matured pollen fertilized ovules, and seeds were produced. Genetic tests with seedlings obtained from pollinations with in-vitro-matured pollen from a transgenic plant revealed normal Mendelian segregation of two marker genes, the neomycin-phosphotransferase II gene and the nopaline-synthase gene. These results are of interest with respect to the control of self-incompatibility, cytoplasmic male sterility and pollen-allergen formation, and it offers an alternative route for gene transfer in those plants which cannot be regenerated in vitro.

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