Oxygen transport is a major limitation in large-scale mammalian cell culture. The effects of the dissolved oxygen concentration (DO; from 0.1 to 100% saturation with air) on Sp2/0-derived mouse hybridomas were investigated using continuous culture. The steady-state concentration of viable cells incr
✦ LIBER ✦
In situ recalibration of probes of dissolved gas concentrations in bioreactors without effect on metabolism and growth
✍ Scribed by J. J. Lønsmann Iversen
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 365 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0006-3592
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## Abstract The mechanisms of iron (Fe) and transferrin (Tf) uptake by the human melanoma cell line, SK‐MEL‐28, have been investigated using chelators and metabolic probes. These data provide evidence for two saturable processes of Fe uptake from Tf, namely, specific receptor‐mediated endocytosis a