In situ indirect fluorescent antibody: A new specific test to detect ongoing chagasic infections

✍ Scribed by Antonio Marcos de Apparecida Levy; Elias Boainain; Judith Kardos Kloetzel

• Book ID: 102657228
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 574 KB
• Volume: 10
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/(sici)1098-2825(1996)10:2<98::aid-jcla7>3.0.co;2-h

✦ Synopsis

In situ immunofluorescence assay (ISIFA) was developed for the selection fortreatment of chronically infected chagasic patients and their follow-up. Trypomastigotes flash fixed in situ on microscopic slides with 0.025% glutaraldehyde were used as antigen. ISlFA results were compared with complement-mediated lysis (CoML), membrane immunofluorescence (MblFA), immunofluorescence assay (IFA) with epimastigotes, and xenodiagnosis (XENO). ISlFAwas able to distinguish nontreated chagasic patients (geometric mean titer [GMT] = 180) and treatment failures (GMT = 160) from those considered successfully treated (GMT = 25). ISlFA revealed a high sensitivity and titers of 80 or higher detected 98.6% of patients with active infections, even in those with negative XENO, CoML, or MblFA. Specificity evaluated in 63 sera from other infections, including leishmaniasis and autoimmune diseases, was 98%. IFA used in routine diagnostic procedures exhibited similar results in all groups, irrespective of therapy. o 1996 Wiley-Liss, Inc.