✦ LIBER ✦

In situ immobilization of alkaline protease during inverse suspension polymerization of polyacrylamide and poly(acrylamide-co-methacrylic acid) hydrogel beads

✍ Scribed by S. Kiatkamjornwong; N. Siwarungson; A. Nganbunsri

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 565 KB
Volume: 73
Category: Article
ISSN: 0021-8995
DOI: 10.1002/(sici)1097-4628(19990912)73:11<2273::aid-app25>3.0.co;2-f

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✦ Synopsis

We report the in situ immobilization of alkaline protease in highly porous polyacrylamide and poly(acrylamide-co-methacrylic acid) hydrogel beads synthesized by inverse suspension polymerization using ammonium persulfate, N,N,NЈ,NЈ,-tetraethylmethylenediamine as the redox initiator. The influences of reaction variables on the polymerization, conversion, enzymatic activity, water absorption, and LCST properties were studied in detail. Evaluation of the enzymatic activities on various substrates was carried out for potential applications in the detergent industry.