In Situ Hybridization with Strand-Specific DNA Probes Generated by a Two-Step Polymerase Chain Reaction Procedure

Detection of Y-bearing porcine spermatoz

Detection of Y-bearing porcine spermatozoa by in situ hybridization using digoxigenin-labeled, porcine male-specific DNA probe produced by polymerase chain reaction

✍ Tatsuo Kawarasaki; Tetsuya Kohsaka; Masaru Sone; Mitsutoshi Yoshida; Kimio Bamba 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 492 KB

This study was carried out to determine whether Y-bearing porcine spermatozoa could be detected by in situ hybridization using a digoxigenin (Dig)-labelled DNA probe specific to the Y chromosome produced by polymerase chain reaction (PCR). A conventional PCR (with Dig-dUTP) was performed using a set