In situ hybridization of chromosome 6 on fine-needle aspirates from breast carcinomas: Comparison of numerical abnormalities and ER/PgR status and staining pattern
✍ Scribed by Torill Sauer; Kahsai Beraki; Peter Wilhelm Jebsen; Eli Ormerod; Oddvar Naess
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 129 KB
- Volume
- 16
- Category
- Article
- ISSN
- 8755-1039
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✦ Synopsis
The estrogen receptor (ER) gene is located on chromosome 6. The aim of our study was to investigate whether numerical chromosomal aberrations were reflected in estrogen/progesterone receptor (PgR) status and staining pattern. Fine-needle aspirates from 51 breast carcinomas were investigated immunocytochemically for ER/PgR and by in situ hybridization technique using digoxigenin-labeled alpha-satellite probe for chromosome 6. Cases with > or = 70% two-signal nuclei were regarded as disome; the remaining tumors showed aneusomy with a variable number of signals. Aneusomy was found in 32 tumors (63%), whereas 19 (37%) had a normal number of chromosome 6. Chromosomal gain occurred in all aneusome cases except one. ER- and/or PgR-positive tumors had an equal distribution of disomy and aneusomy. Variable ER staining pattern or ER and/or PgR negativity was associated with numerical aberrations in chromosome 6 in 76% of the tumors. Cancers with uniform ER staining pattern all had normal chromosome number.