In situ hybridization mapping of the growth hormone receptor (GHR) gene assigns a linkage group (C9, FSA, GHR, and S0105) to Chromosome 16 in pigs
✍ Scribed by B. P. Chowdhary; H. Ellegren; M. Johansson; L. Andersson; I. Gustavsson
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 336 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0938-8990
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✦ Synopsis
The completeness and utility of a gene map depend primarily on the uniform distribution of informative markers along the genome. Although gene mapping efforts during the past few years have resulted in a significant coverage of the porcine genome (Andersson et al. 1993), certain chromosomes/chromosomal regions still lack assigned markers. Chromosome (Chr) 16 is one of the chromosomes to which no genetic marker has yet been mapped. Recently, sequences homologous to the nucleolar organizer region (NOR) were localized by fluorescence in situ hybridization to the heterochromatic band in the middle of this chromosome (Bosma et al. 1991). There is, however, no confirmatory evidence that the hybridization site represents a NOR region, because the silver nitrate method (Bloom and Goodpasture 1976) does not demonstrate any staining on this chromosome.
In the present study, we describe the chromosomal localization of the growth hormone receptor (GHR) gene to Chr 16 in pigs, using radioactive in situ hybridization (ISH). In addition, we also report the identification and subsequent assignment of a linkage group comprising the loci for GI-IR, complement factor 9 (C9) and follistatin (FSA), and the microsatellite S0105, to this chromosome.