In situ hybridisation analysis of a homogeneously staining region at 11q23–24 in an acute myeloid leukaemia (M5) using yeast artificial chromosomes
✍ Scribed by Elisabeth Nacheva; Lyndal Kearney; Mark Bower; Tracy Chaplin; Edward Douek; Dr. Soma Das; Bryan D. Young
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 481 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
An example of a homogeneously staining region (hsr), occurring in an acute myeloid leukaemia (M5) on chromosome I I in the region of bands q23-q24, has been analysed. In situ hybridisation using yeast artificial chromosome (YAC) DNA demonstrated that the amplification did not include the CD3 gene cluster and did not affect the human trithorax gene known to be disrupted by translocations at I I q23. In contrast, the amplification was shown to include the sequence D I I S543 which has been previously mapped t o chromosome band I 1924. High resolution analysis using confocal microscopy allowed the individual amplicons t o be visualised, and it was shown that the hsr consisted of an 8-fold amplification of the region surrounding the probe D I I S543. From previous estimates of human chromosome size it was possible t o calculate that the hsr was composed of amplicons approximately I0 megabases in length. It was concluded that the region amplified did not extend as far as the translocation breakpoints occurring at I lq23 in acute leukaemias. Genes Chrorn Cancer 7~123-127 (1993).