In situ enzymatic digestion of oligopeptides on a direct insertion probe tip combined with fast atom bombardment and tandem mass spectrometry

✍ Scribed by Marianna Mák; József Tamás

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 342 KB
• Volume: 28
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/oms.1210280512

✦ Synopsis

The applicability of an approach to establish the sequence of oligopeptides involving on-probe-tip enzymatic fragmentation and subsequent direct fast atom bombardment (FAB) mass spectrometry (MS) and FABMS/MS of the subpeptide mixture was tested using two model peptides consisting of 19 and 25 amino acid residues. The methad was found to be both time and sample sparing and flexible enough to use even subsequent digests, if necessary, with enzymes of different specificity. It provided in both cases complete information, not only for the sequence of enzymic fragments but also for their order in the original oligopeptide. Consequently, this approach to the sequence analysis of oligopeptides can be a method of choice when the FABMS/MS technique itself is unsuitable for obtaining detailed structural information. The method seems to be applicable to enzymically digestible peptides with an upper mass limit of about 3500.