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In situ ELISA for the evaluation of antiviral compounds effective against human cytomegalovirus

✍ Scribed by Walter A. Tatarowicz; Nell S. Lurain; Kenneth D. Thompson

Book ID
119115049
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
627 KB
Volume
35
Category
Article
ISSN
0166-0934

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Two prokaryotically expressed fusion proteins encompassing amino acids 484-650 (AD-I) and 27-100 (AD-2) of glycoprotein gp58/116 of human cytomegalovirus (HCMV) were purified from E. coli lysates and used in ELISA to determine antibody levels in human sera. The specificity of the test was establishe