In-house validation of a high-performance liquid chromatography analytical method for quantification of ochratoxin A in unfermented grape juice

✍ Scribed by Ma Teresa Murillo; Elena González-Peñas; Susana Amézqueta; Adela López de Cerain

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 136 KB
• Volume: 87
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/jsfa.2980

✦ Synopsis

Abstract

A validated high‐performance liquid chromatography (HPLC) method with fluorescence detection for the quantitative analysis of ochratoxin A (OTA) in unfermented grape juice is described. Five millilitres of unfermented grape juice was mixed with 45 mL of PBS, and the pH was adjusted to 7.2. Then the mixture was filtered under vacuum through a glass microfibre filter and cleaned up with immunoaffinity columns prior to analysis by HPLC. Validation of the analytical method was based on the following criteria: selectivity, linearity, limit of detection and quantification, precision (within‐day and between‐day variability) and recovery and uncertainty estimation. Detection and quantification limits obtained were 0.02 µg L^−1^ and 0.05 µg L^−1^ respectively. The percentage recovery was 91.5% (RSD = 3.9). This method was applied to the measurement of 30 veraison stage unfermented grape juice samples. Copyright © 2007 Society of Chemical Industry