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In-Gel Stable-Isotope Labeling (ISIL): A Strategy for Mass Spectrometry-Based Relative Quantification

✍ Scribed by Asara, John M.; Zhang, Xiang; Zheng, Bin; Christofk, Heather H.; Wu, Ning; Cantley, Lewis C.


Book ID
126438374
Publisher
American Chemical Society
Year
2006
Tongue
English
Weight
575 KB
Volume
5
Category
Article
ISSN
1535-3893

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## Abstract In the present work we report on a novel and fast protocol for accurate bottom‐up protein quantification that overcomes the drawbacks of in‐gel digestion and MALDI analysis, while maintaining their benefits. It relies on the following steps: (i) gel electrophoresis separation of protein