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`In gel' cleavage with cyanogen bromide for protein internal sequencing

✍ Scribed by Osvaldo L Córdoba; Susana B Linskens; Evangelina Dacci; José A Santomé


Book ID
117331455
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
714 KB
Volume
35
Category
Article
ISSN
0165-022X

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We examined the different steps necessary for the enzymatic digestion of proteins in the polyacrylamide matrix after gel electrophoresis. As a result, we developed an improved method for obtaining peptides for internal sequence analysis from 1-2 micrograms of in-gel-digested proteins. The long washi