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In-frame length mutations associated with short tandem repeats are located in unassigned open reading frames ofOenotherachloroplast DNA

✍ Scribed by Rolf Nimzyk; Thomas Schöndorf; Wolfgang Hachtel

Book ID: 104735322
Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 791 KB
Volume: 23
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00351505

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✦ Synopsis

Chloroplast DNAs were compared between two closely related species in the subsection Munzia of the genus Oenothera. A restriction fragment length dimorphism (273 bp) within the large inverted repeats was localized to an unassigned open reading frame that is homologous to ORF 2280 of tobacco chloroplast DNA. This dimorphism is due to different copy numbers of various short tandem repeated sequences, with each repeat unit specifying an in-frame addition or deletion. Other small length mutations were detected within an unassigned reading frame that appears to be homologous to the tobacco ORF 1244, and in the non-coding sequence upstream of that frame. These insertions and/or deletions are all associated with short direct repeats that lie in tandem.

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Deletions/insertions, short inverted rep

Deletions/insertions, short inverted repeats, sequences resembling att-lambda, and frame shift mutated open reading frames are involved in chloroplast DNA differences in the genus Oenothera subsection Munzia

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