✦ LIBER ✦

In-cell protein NMR and protein leakage

✍ Scribed by Christopher O. Barnes; Gary J. Pielak

Book ID: 105358180
Publisher: John Wiley and Sons
Year: 2010
Tongue: English
Weight: 307 KB
Volume: 79
Category: Article
ISSN: 0887-3585
DOI: 10.1002/prot.22906

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In‐cell nuclear magnetic resonance spectroscopy is a tool for studying proteins under physiologically relevant conditions. In some instances, however, protein signals from leaked protein are observed in the liquid surrounding the cells. Here, we examine the expression of four proteins in Escherichia coli. We describe the controls that should be used for in‐cell NMR experiments and show that leakage is likely when the protein being studied exceeds ∼20% of the total cellular protein. Proteins 2011. © 2010 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

In-cell NMR for protein-protein interact

In-cell NMR for protein-protein interactions (STINT-NMR)

✍ Burz, David S; Dutta, Kaushik; Cowburn, David; Shekhtman, Alexander 📂 Article 📅 2006 🏛 Nature Publishing Group 🌐 English ⚖ 307 KB

Cell-free Protein Synthesis in an Autoin

Cell-free Protein Synthesis in an Autoinduction System for NMR Studies of Protein–Protein Interactions

✍ Kiyoshi Ozawa; Slobodan Jergic; Jeffrey A. Crowther; Phillip R. Thompson; Gene W 📂 Article 📅 2005 🏛 Springer Netherlands 🌐 English ⚖ 311 KB

Protein adsorption and leakage in carrie

Protein adsorption and leakage in carrier–enzyme systems

✍ Renate Ulbrich; Ralph Golbik; Alfred Schellenberger 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 701 KB

Cell-free expression and selective isoto

Cell-free expression and selective isotope labelling in protein NMR

✍ David Staunton; Robin Schlinkert; Giulia Zanetti; Simon A. Colebrook; Iain D. Ca 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 852 KB

Isotope labelling is a very powerful tool in NMR studies of proteins and has been employed in various ways for over 40 years. 15N and 13C incorporation, using recombinant expression systems, is now commonplace because heteronuclear experiments assist with the fundamental problems of peak resolution

Clara cell protein and surfactant protei

Clara cell protein and surfactant protein

✍ D. Steiner; S. Jeggli; A. Tschopp; A. Bernard; A. Oppliger; S. Hilfiker; P. Hotz 📂 Article 📅 2005 🏛 Springer-Verlag 🌐 English ⚖ 304 KB

Cell-free protein synthesis of perdeuter

Cell-free protein synthesis of perdeuterated proteins for NMR studies

✍ Touraj Etezady-Esfarjani; Sebastian Hiller; Cristina Villalba; Kurt Wüthrich 📂 Article 📅 2007 🏛 Springer Netherlands 🌐 English ⚖ 1005 KB