In-111Pseudomonas aeruginosa: A simple method of labeling live bacteria with a gamma-emitting radioisotope

✍ Scribed by Kristine Bettin; Melody O'Connor Allen; Dale N. Gerding; Lee Forstrom; Rex Shafer

• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 295 KB
• Volume: 12
• Category: Article
• ISSN: 0340-6997
• DOI: 10.1007/bf00251987

✦ Synopsis

We describe a simple and reliable technique for labeling Pseudomonas aeruginosa with a readily available commercial preparation of indium-111 (111In) oxine. Labeling of a heavy bacterial suspension with 500 mu Ci of commercially prepared 111In-oxine resulted in a yield of 0.0004 mu Ci of cell-associated 111In per 10(6) colony-forming units (CFU). The label was 88% bacterially associated and did not effect viability of the organism. Radiolabeling a gram-negative organism with 111In-oxine provides a non-toxic, stable gamma-emitting bacterial tracer.