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Improving yield and quality of recombinant proteins in Escherichia coli by stress minimisation and mutant selection

✍ Scribed by Y. Sevastsyanovich; S. Alfasi; L. Griffiths; T. Overton; J. Cole


Book ID
108217216
Publisher
Elsevier
Year
2009
Tongue
English
Weight
80 KB
Volume
25
Category
Article
ISSN
1871-6784

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## Abstract Many enzymes or fluorescent proteins produced in __Escherichia coli__ are enzymatically active or fluorescent respectively when deposited as inclusion bodies. The occurrence of insoluble but functional protein species with native‐like secondary structure indicates that solubility and co