Improving X!Tandem on Peptide Identification from Mass Spectrometry by Self-Boosted Percolator
✍ Scribed by Yang, Pengyi; Ma, Jie; Wang, Penghao; Zhu, Yunping; Zhou, Bing B.; Yang, Yee Hwa (Jean)
- Book ID
- 117908653
- Publisher
- IEEE
- Year
- 2012
- Tongue
- English
- Weight
- 706 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1545-5963
No coin nor oath required. For personal study only.
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Synthetic proteins with unusual architecture are obtained through chemoselective ligation, a method based on the condensation of unprotected peptides under mild aqueous conditions. The last step of a new procedure leading to a tri-branched conjugate consists of the chemoselective ligation reaction b
An on-line method has been developed for the derivatization and coupled liquid chromatography (LC)/electrospray ionization (ESI) MS analysis of peptides at the femtomol level. Peptides are reacted with N-succinimidyl-2(3-pyridyl)acetate (SPA) in buffered aqueous medium at pH 7 following loading on a
## Abstract Matrix‐assisted laser desorption ionization (MALDI), Peptide Mass Fingerprinting (PMF) and MALDI‐MS/MS ion search (using MASCOT) have become the preferred methods for high‐throughput identification of proteins. Unfortunately, PMF can be ambiguous, mainly when the genome of the organism
## Abstract Matrix‐assisted laser desorption ionization (MALDI), Peptide Mass Fingerprinting (PMF) and MALDI‐MS/MS ion search (using MASCOT) have become the preferred methods for high‐throughput identification of proteins. Unfortunately, PMF can be ambiguous, mainly when the genome of the organism