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Improving Reproducibility and Sensitivity in Identifying Human Proteins by Shotgun Proteomics

โœ Scribed by Resing, Katheryn A.; Meyer-Arendt, Karen; Mendoza, Alex M.; Aveline-Wolf, Lauren D.; Jonscher, Karen R.; Pierce, Kevin G.; Old, William M.; Cheung, Hiu T.; Russell, Steven; Wattawa, Joy L.; Goehle, Geoff R.; Knight, Robin D.; Ahn, Natalie G.


Book ID
120410846
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
203 KB
Volume
76
Category
Article
ISSN
0003-2700

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We describe approaches to improve the detection of proteins by postharvest alkylation and subsequent radioactive labeling with either [3H]iodoacetamide or 125I. Database protein sequence analysis suggested that cysteine is not suitable for detection of the entire proteome, but that cysteine alkylati