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Improving rAAV production and purification: towards the definition of a scaleable process

✍ Scribed by Véronique Blouin; Nicole Brument; Estelle Toublanc; Isabelle Raimbaud; Philippe Moullier; Anna Salvetti

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
151 KB
Volume
6
Category
Article
ISSN
1099-498X

No coin nor oath required. For personal study only.

✦ Synopsis

Numerous in vivo studies have demonstrated that recombinant AAV-2 vectors (rAAV-2) can efficiently transduce many tissues and lead to stable gene expression 12. These encouraging results have led to a rapid development of clinical trials involving the use of rAAV-2 vectors 3. However, the obtainment of large-scale rAAV-2 vector stocks for clinical assay is still hampered by the conventional production and purification methods that are not efficient and difficult to scale up. This review will describe the current methods available for rAAV-2 vector production and purification and show the advancements achieved, in particular in our group, to develop new scalable procedures, suiting GMP requirements.

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