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Improving cell-free protein synthesis for stable-isotope labeling

โœ Scribed by Takayoshi Matsuda; Seizo Koshiba; Naoya Tochio; Eiko Seki; Noriyuki Iwasaki; Takashi Yabuki; Makoto Inoue; Shigeyuki Yokoyama; Takanori Kigawa

Publisher
Springer Netherlands
Year
2007
Tongue
English
Weight
216 KB
Volume
37
Category
Article
ISSN
0925-2738

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๐Ÿ“œ SIMILAR VOLUMES

Cell-free synthesis and amino acid-selec
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โœ Takanori Kigawa; Yutaka Muto; Shigeyuki Yokoyama ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 536 KB

For the application of multidimensional NMR spectroscopy to larger proteins, it would be useful to perform selective labeling of one of the 20 amino acids. For some amino acids, however, amino acid metabolism drastically reduces the efficiency and selectivity of labeling in in vivo expression system

Cell-free expression and selective isoto
Cell-free expression and selective isotope labelling in protein NMR
โœ David Staunton; Robin Schlinkert; Giulia Zanetti; Simon A. Colebrook; Iain D. Ca ๐Ÿ“‚ Article ๐Ÿ“… 2006 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 852 KB

Isotope labelling is a very powerful tool in NMR studies of proteins and has been employed in various ways for over 40 years. 15N and 13C incorporation, using recombinant expression systems, is now commonplace because heteronuclear experiments assist with the fundamental problems of peak resolution