✦ LIBER ✦

Improving bioaffinity NMR spectra by means of zero-quantum dephasing

✍ Scribed by Thomas Weimar

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 68 KB
Volume: 38
Category: Article
ISSN: 0749-1581
DOI: 10.1002/(sici)1097-458x(200004)38:4<315::aid-mrc647>3.0.co;2-0

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✦ Synopsis

Bioaffinity NMR is based on the observation of transferred NOE (trNOE) effects which stem from bioactive components in a mixture containing a library of small compounds and a protein target. Zero-quantum coherence signals can interfere with the trNOE cross peaks and, in the worst case, prevent the detection of a bioactive compound in a library. The dephasing of these zero-quantum coherence signals during the experiment significantly improves the bioaffinity NMR spectra obtained and greatly reduces the risk of misinterpretations.