Binding of ibuprofen (IB) enantiomers to human serum albumin (HSA) was studied using a chiral fluorescent derivatizing reagent, which enabled the measurement of IB enantiomers at a concentration as low 5 x 10(-8) M. Scatchard analyses revealed that there were two classes of binding sites for both en
β¦ LIBER β¦
Improving Antisense Oligonucleotide Binding to Human Serum Albumin: Dramatic Effect of Ibuprofen Conjugation
β Scribed by Muthiah Manoharan; Gopal B. Inamati; Elena A. Lesnik; Namir B. Sioufi; Susan M. Freier
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 186 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1439-4227
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The binding of racemic, (R)([) and (S)(])-ibuprofen (IBP) to human serum albumin (HSA) was studied using NMR spectroscopy. Having saturated the tight binding sites, the extent of weak binding was then investigated. The molecular di β usion coefficients of IBP in HSA solution at di β erent concentratio