Improvement of the total turnover number and space-time yield for chloroperoxidase catalyzed oxidation
✍ Scribed by K. Seelbach; M. P. J. van Deurzen; F. van Rantwijk; R. A. Sheldon; U. Kragl
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 168 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Chloroperoxidase from Caldariomyces fumago was applied for the oxidation of indole to oxindole using hydrogen peroxide as the oxidant in aqueous t-butyl alcohol medium. Different ways of adding the oxidant, various reactor types, and the use of a hydrogen peroxide-stat were compared, resulting in a 20-fold increase of the total turnover number (ttn) and space-time yield (sty). The highest ttn of >860,000 was obtained in a fed-batch reactor, whereas the highest sty of 120 g/(L ⅐ d) was reached in a continuously operated enzyme membrane reactor. The results were compared to other enzyme systems already established for the synthesis of amino acids and carbohydrates.