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Improvement of the dot-blot-SNP technique for efficient and cost-effective genotyping

✍ Scribed by Sachiko Shiokai; Kenta Shirasawa; Yutaka Sato; Takeshi Nishio

Book ID
106469138
Publisher
Springer Netherlands
Year
2009
Tongue
English
Weight
324 KB
Volume
25
Category
Article
ISSN
1380-3743

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Two techniques, ELISA and dot-blot, were applied to the qualitative detection of very low levels of whey proteins in liver paths. The use of an avidin-biotin ampli~cation system for both methods led to a useful improvement of the detection limit. The detection level which was 4 g kg-' with the clas