Improvement of lipase stability in the presence of commercial triglycerides
โ Scribed by Domenico Pirozzi
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 301 KB
- Volume
- 105
- Category
- Article
- ISSN
- 1438-7697
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๐ SIMILAR VOLUMES
Lipoprotein lipase (LPL) and hepatic triglyceride lipase (HL) were biotinylated using N-hydroxysuccinamide ester of biotin (25-fold molar excess) which was incorporated into the lysine amino groups of the enzyme protein. By assessing enzyme activity and heparin-agarose affinity a biotinylation proto
## Abstract The addition of a very small concentration of a detergent (in many instances under the critical micellar concentration (cmc)) has been found to greatly increase the activity of immobilized lipases, using those from __Pseudomonas fluorescens__ (PFL) and __Candida antarctica__ (isoform B)