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Improvement of biological and pharmacokinetic features of human interleukin-11 by site-directed mutagenesis

✍ Scribed by Yuni Jung; Haesook Ahn; Dong-Sik Kim; Yu Ri Hwang; Seong-Hyun Ho; Jong-Mook Kim; Sujeong Kim; Suyong Ma; Sunyoung Kim

Book ID
116301335
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
630 KB
Volume
405
Category
Article
ISSN
0006-291X

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## Abstract By directed evolution and subsequent site‐directed mutagenesis, cold‐adapted variants of WF146 protease, a thermophilic subtilase, have been successfully engineered. A four‐amino acid substitution variant RTN29 displayed a sixfold increase in caseinolytic activity in the temperature ran