Improvement of assay sensitivity in immunoassay: chemiluminescence immunoassay for estradiol as a model

✍ Scribed by J. Bouve; J. De Boever; D. Leyseele; D. Vandekerckhove; F. Kohen

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 536 KB
• Volume: 255
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(91)80077-7

✦ Synopsis

Recewed 29th Apnll991) Vanatums of sohd-phase format and ensumg assay sensltivlty were compared The assay, a chemdumm~cc ll~~unoassay for estrad~ol, uses a homologous combmation of a monoclonal antibody to estr~ol-6-uuboxymethyloxune-bovme sermn albumm and estrtiol-6-carboxymethylo~e-ammobutylethybsolummol (E,-ABEI) as chemdmescent marker When polyacryhumde beads were uw as the sohd phase for the monoclonal antIbody. the detectmn tit (4 9 pg E, per cuvette) was moderate When the monoclonal antibody was bound to rmcrotitre plates, the detection lmut dmeased to 0 71 pg E, per well Delayed ad&bon of the E&BE1 marker Improved the detection bt to 0 37 pg Ez per well An altematlve system was also evaluated rmcrot~tre plates ware coated with a second ant~body and monoclonal antibody was mcubated with the other reagents This assay format, m combmatmn urlth delayed addition of the marker, resulted m a detection ht of 0 25 pg E2 per well