Improvement of a Continuous Spectrophotometric Method for Determining the Monophenolase and Diphenolase Activities of Mushroom Polyphenol Oxidase
✍ Scribed by Espín, Juan Carlos; Morales, Mercedes; García-Ruiz, Pedro Antonio; Tudela, José; García-Cánovas, Francisco
- Book ID
- 121310351
- Publisher
- American Chemical Society
- Year
- 1997
- Tongue
- English
- Weight
- 197 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0021-8561
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A continuous spectrophotometric method for the rapid determination of diphenolase activity of tyrosinase is described. It uses 3,4-dihydroxymandelic acid (DOMA) as the substrate of tyrosinase and measures the final product, 3,4-dihydroxybenzaldehyde (DOBA). The spectrum of this product shows a batho
A continuous spectrophotometric method for the rapid determination of monophenolase activity of tyrosinase is described. This method is based on the coupling reaction between 3-methyl-2-benzothiazolinone hydrazone (MBTH) and the quinone products of the oxidation of various monophenols in the presenc