Rational improvement of cell-free protei
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Anders Pedersen; Kristofer Hellberg; Johan Enberg; B. Göran Karlsson
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Article
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2011
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Elsevier
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English
⚖ 415 KB
Experimental design principles were applied on cell-free protein synthesis to optimize performance with regard to the expression yield and the incorporation efficiency of amino acid precursors. A versatile screening platform based on batch-mode cell-free expression and central composite design was u