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Improved Visualization of Vertebrate Nuclear Pore Complexes by Field Emission Scanning Electron Microscopy

✍ Scribed by Lihi Shaulov; Amnon Harel


Book ID
113919162
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
958 KB
Volume
20
Category
Article
ISSN
0969-2126

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The lateral resolution attained by scanning force microscopy (SFM) on disordered membrane protein structures is often limited. We have imaged one such structure -the nuclear pore complex, on chemically fixed nuclear envelopes isolated from Xenopus oocytes, optimizing procedures to maximize lateral r