Improved technique on reversion of mycelial protoplast of Trichoderma reesei into mycelia

✍ Scribed by J. Anjani Kumari; T. Panda

• Book ID: 104744510
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 714 KB
• Volume: 8
• Category: Article
• ISSN: 1615-7605
• DOI: 10.1007/bf00369843

✦ Synopsis

Important parameters in the regeneration of protoplasts are viability, the capacity to synthesize cell walls and the retention of properties of the parent cell. Mycelial protoplasts of Trichoderma longibrachiatum (Trichoderma reesei) have been regenerated. Factors influencing the regeneration of protoplasts of T. longibrachiatum QM 9414 were found to be, the nature of osmotic stabilizer, the concentration of osmotic stabilizer, pH, temperature, and the composition of regeneration medium. With glucose-mineral regeneration medium, the optimum conditions for protoplasts regeneration were 0.5 M KC1, pH 6.0 and temperature 30~ With Czapek-Dox medium, the optimum conditions were 0.7 M mannitol, pH 6.0 and temperature 30 ~ Maximum regeneration frequency of T. longibrachiatum protoplasts were obtained using glucose-mineral medium.

with the optimization of various factors that influence reversion of mycelial protoplasts of T. longibrachiatum.

Materials and methods

2.1 Organisms

Trichoderma longibrachiatum, QM 9414, and Trichoderma harzianum, NCIM 1185, were obtained from the National Chemical Laboratory, Pune, India.