Improved techniques for the isolation of
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Eric Davies; J.E. Dumont; G. Vassart
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Article
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1977
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Elsevier Science
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English
⚖ 490 KB
A method is presented, based on the use of an extraction buffer containing 200 mM Tris-HCl, pH 8.5, which permits the isolation in high yield of apparently undegraded polysomes from beef thyroid. Upon treatment with detergent, both the 8OOg pellet and the 27,000g pellet yield polysome profiles exhib