✦ LIBER ✦
Improved Synthesis of Oligodeoxyribonucleotides by Solid-Phase Phosphotriester Method Utilizing O6-[2-(p-Nitrophenyl)ethyl]-2′-deoxyguanosine Derivatives
✍ Scribed by André Chollet; Edgar Ayala; Eric H. Kawashima
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- German
- Weight
- 610 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0018-019X
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✦ Synopsis
Abstract
The synthesis of oligodeoxyribonucleotides on a cross‐linked polystyrene solid support utilizing stable mono‐ and dinucleotide phosphotriester building blocks is presented. The use of O^6^[2‐(p‐nitrophenyl)ethyl]‐2′‐deoxyguanosine derivatives yields cleaner DNA fragments by suppressing side reactions. Modifications improving the phosphotriester methodology are presented. The purification methods and analysis of synthetic oligodeoxyribonucleotides are described.