Improved sensitivity of chromogenic substrate assays for urokinase and plasmin
โ Scribed by Robert B Friedman; Hau C Kwaan; Marija Szczecinski
- Book ID
- 119033682
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 508 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0049-3848
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A simple and highly sensitive chromogenic microplate assay for quantification of rat and human plasminogen in plasma samples and subcellular fractions has been developed. The assay is based on a conversion of plasminogen to plasmin, using urokinase as an activator, and a subsequent cleavage of a chr
Phe5(4-nitro)-bradykinin has been identified as a good synthetic substrate to study the kinetics and mechanism of action of the metalloendopeptidase meprin. No convenient substrate for kinetic analysis of the enzyme had been previously described. HPLC analyses indicated that meprin cleaved bradykini