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Improved quantification of in vivo1H NMR spectra by optimization of signal acquisition and processing and by incorporation of prior knowledge into the spectral fitting

✍ Scribed by A. A. De Graaf; W. M. M. J. Bovée

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
851 KB
Volume
15
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

Quantification of localized in vivo brain ^1^H spectra is in general very difficult due to excessive spectral overlap. In addition, intensity distortions may result from the effects of the NMR pulse sequence on the spins. This paper describes an approach to solving these problems. It comprises optimization of the pulse sequence; correction of the experimental lineshape; determination of intensity distortions, of relative line positions, and of linewidths using model solutions; and incorporation of the thus obtained prior knowledge into a nonlinear least‐squares spectral fitting procedure. This approach resulted in greatly improved accuracy, precision, and reliability of the quantitation of our in vivo spectra of rat brain, and enabled us to estimate absolute metabolite concentrations. © 1990 Academic Press, Inc.

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