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Improved protocol for the preparation of chromatin fibres from fixed cells

✍ Scribed by Frank Speleman; Mireille Van Gele; Luc Maertens; Nadine Van Roy

Book ID
114393021
Publisher
Elsevier
Year
1997
Weight
92 KB
Volume
2
Category
Article
ISSN
1366-2120

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✍ Thompson, J. R.; Register, E.; Curotto, J.; Kurtz, M.; Kelly, R. πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 98 KB πŸ‘ 2 views

Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-f