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Improved Protein Recovery in Reversed-Phase Liquid Chromatography by the Use of Ultrahigh Pressures

โœ Scribed by Eschelbach, John W.; Jorgenson, James W.

Book ID
126261319
Publisher
American Chemical Society
Year
2006
Tongue
English
Weight
175 KB
Volume
78
Category
Article
ISSN
0003-2700

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Fast protein separation by reversed-phase high-performance liquid chromatography on octadecylsilyl-bonded nonporous silica gel: II. Improvement in recovery of hydrophobic proteins
โœ Hiroko Itoh; Noriyuki Nimura; Toshio Kinoshita; Norikazu Nagae; Mitsugu Nomura ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 388 KB

Recovery of hydrophobic proteins from an RP-HPLC column was improved using a fast-separation RP-HPLC system operated at room temperature. Hydrophobic proteins such as ovalbumin could be adequately eluted from a nonporous octadecylsilyl (C18) spherical silica gel with a particle diameter of 20 micron