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Improved procedures for the synthesis of phosphomevalonate and for the assay and purification of pig liver phosphomevalonate kinase

โœ Scribed by Lee, Choy Soong; O'Sullivan, William J.

Book ID
122821528
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
583 KB
Volume
839
Category
Article
ISSN
0304-4165

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A new assay system for total phosphorylase activity (a + b form) from rabbit liver was developed by the use of 20% (v/v) 1,2-dimethoxyethane and 20 mM AMP, which provided high activation of the b form. In the absence of 1,Zdimetboxyethane and AMP, phosphorylase a had 100% of the activity (50 units/m