Improved plant regeneration in maize callus cultures after pretreatment withdl-alpha-difluoromethylarginine
✍ Scribed by Antonio F. Tiburcio; Xavier Figueras; Imma Claparols; Mireia Santos; José Ma Torné
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 673 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
The influence of a three-month-long pretreatment with oL-alpha-difluoromethylarginine (DFMA), an irreversible suicide inhibitor of arginine decarboxylase activity (ADC; EC 4.1.1.19), on plant regeneration, protein and polyamine (PA) composition of Zea mays callus cultures has been investigated. A four-fold increase in the number of regenerated plants is obtained after pretreatment with 0.5 mM DFMA. In addition, the regeneration frequency increases 3-fold in the treated calluses and the plants regenerated from such cultures are more developed than the untreated controls. The data obtained on protein and PA contents suggest that a senescence effect is exerted on the calluses grown in the presence of DFMA. However, after DFMA removal a rejuvenation effect occurs on the calluses that may explain the improvement of morphogenic capacity. This study indicates that DFMA pretreatment can be used to increase regeneration efficiency from maize callus cultures.